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Superoxide production try perceived in the bloodstream and BALF using the CMH twist probe. Blood or BALF examples had been accumulated away from PBS- and you will Bleo-treated mice and you can incubated immediately which have CMH. The newest examples have been gone to live in the newest PTFE tube and flash suspended, and EPR investigation is actually obtained at the 77 K. The newest concentration of nitroxide (CM.) collected inside blood incubated with CMH (0.2 mM) in the 37 degree to own ten min (Profile 4A).
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The new atomic nucleus in addition to have spin, but protons and neutrons are a lot hefty than just electrons (from the 1836 minutes), and the magnetic dipole second try inversely proportional on the size. So the nuclear magnetized dipole momentum is much smaller than you to definitely of your whole atom. That it brief magnetized dipole are later counted by Stern, Frisch and you will Easterman. The brand new trend will be told me to your spatial quantization of the twist moment from momentum. Inside the atoms the new electrons is actually matched such that you to revolves upward and another downwards, neutralizing the result of the twist to your action of the atom total.